Which of the following is an example of degradative plasmid?
Degradative plasmids, which enable the digestion of unusual substances, e.g. toluene and salicylic acid. Virulence plasmids, which turn the bacterium into a pathogen. e.g. Ti plasmid in Agrobacterium tumefaciens.
What is an example of a resistance plasmid?
Quinolone resistance genes are frequently located on the same plasmid as the ESBL genes. Examples of resistance mechanisms include different Qnr proteins, aminoglycose acetyltransferase aac(6′)-Ib-cr that is able to hydrolyze ciprofloxacin and norfloxacin, as well as efflux transporters OqxAB and QepA.
Which plasmid allows for antibiotic resistance?
Plasmids are small DNA circles outside the bacterial chromosome. Several antibiotic resistance genes can be present on the same plasmid. In this example, they are called res A, res B and res C. Res A gives resistance to antibiotic A, res B to antibiotic B and so on.
Which of the following Pseudomonas plasmid is involved in degradative pathways?
TOL plasmids in Pseudomonas putida encode the metabolic pathways for the degradation of toluene, xylenes, and their alcohol and carboxylate derivatives (1).
Is pBR322 is a natural plasmid?
pBR322 DNA is a commonly used plasmid cloning vector in E. coli (1). The molecule is a double-stranded circle 4,361* base pairs in length (2). pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol.
Is E. coli resistant to streptomycin?
Abstract. Escherichia coli K-12 carrying the R-factor R1 or R6K is resistant to streptomycin. The resistance is due to R-factor-coded enzymes that metabolize the drug.
What is the ampicillin resistance gene?
Ampicillin is commonly used as a selection marker since it binds to and inhibits the action of several enzymes that are involved in the synthesis of the cell wall. The ampicillin-resistant gene (ampR), on the other hand, catalyzes the hydrolysis of the B-lactam ring of ampicillin and naturally detoxifies the drug.
What are multidrug resistant plasmids?
Multidrug resistance in bacteria occurs by the accumulation, on resistance (R) plasmids or transposons, of genes, with each coding for resistance to a specific agent, and/or by the action of multidrug efflux pumps, each of which can pump out more than one drug type.
Which plasmid is responsible for degradation of toluene?
putida PaW15, which carries a catabolic plasmid (the TOL plasmid) that encodes toluene degradation genes.
Which is an example of catabolic plasmid?
The very first catabolic plasmid to be isolated was the CAM plasmid of Pseudomonas putida; this plasmid controlled the oxidation of the naturally occurring terpene, camphor (Rheinwald et al., 1973). More surprising was the subsequent discovery that plasmids also encoded the degradation of synthetic molecules.
Which antibiotic resistance is present in pBR322 Mcq?
7. How many sets of antibiotic resistance does the plasmid Pbr322 carry? Explanation: The plasmid contains two sets of antibiotic resistance genes on coding for the ampicillin resistance and the other for tetracycline resistance. 8.
What is a degradative reaction?
Degradation reactions reduce the number of atoms in a molecule through a specific type of chemical transformation.
Is Staphylococcus aureus resistant to streptomycin?
Antibiotic susceptibility of S. Of the total 47 positive S. aureus isolates, the percentage of isolates resistant to Oxacillin (OX), Ampicillin (AMP), Ciprofloxacin (CIP), Tetracycline (TET), Streptomycin (SMN), Erythromycin, (ERY) and Sulfamethoxazole/Trimethoprim (SXT) was 17, 13, 9, 28, 89, 13 and 11% respectively.
Is ampicillin resistant to E. coli?
Results of our research have proved that E. coli showed major antimicrobial resistance to ampicillin and trimethoprim-sulfamethoxazole, and minor resistance to gentamicin.
Is ampicillin resistant to E coli?
Is E coli resistant to tetracycline?
Tetracycline is not used to treat E. coli infections in humans, but resistance to tetracycline is still common in E. coli (4, 7), which suggests that resistance has been selected by a bystander effect on commensal E. coli, during treatment of other pathogens in humans or animals.