What is drug susceptibility testing?

Susceptibility testing is used to determine which antimicrobials will inhibit the growth of the bacteria or fungi causing a specific infection. The results from this test will help a health care practitioner determine which drugs are likely to be most effective in treating a person’s infection.

What is line probe assay for TB?

LPA is a rapid technique based on polymerase chain reaction (PCR) that is used to detect Mycobacterium tuberculosis (MTB) complex as well as drug sensitivity to rifampicin (RPM) and isoniazid (INH) through the Revised National Tuberculosis Control Programme (RNTCP) of India.

What is DST culture?

Drug susceptibility testing (DST) of Mycobacterium tuberculosis is generally carried out after a culture is isolated from a clinical specimen. This takes a long time, first to isolate a culture and then to perform drug susceptibility testing (indirect DST).

Which media is used for drug sensitivity testing of tubercle bacilli?

Resazurin microtiter plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis.

How is drug susceptibility test done?

The LB uses an indirect proportion method for testing M. tuberculosis complex mycobacteria to 12 drugs at 35C on Middlebrook 7H10 agar. The test requires 1 month to complete.

What is SL LPA and FL LPA?

They can be used for testing of culture isolates (indirect testing), as well as direct testing of acid fast bacilli (AFB) smear microscopy positive specimens (FL-LPA), and both smear positive and smear negative sputum specimens (SL-LPA) (6, 7).

Is GeneXpert a gold standard?

(Table 4). Using Culture MGIT as the gold standard, the GeneXpert correctly identified M. tuberculosis in 95 of 121 Culture MGIT positive samples while LPA correctly identified M. tuberculosis in 119 of 121 Culture MGIT positive samples.

What is DST in TB treatment?

The effective management of TB and MDR-TB relies upon rapid diagnosis and effective treatment of resistant infections. Culture-based phenotypic drug susceptibility testing (DST) methods are currently the gold standard for drug resistance detection.

What is FL LPA?

In 2008, the World Health Organization (WHO) endorsed the use of the first line (FL) line probe assay (LPA), the GenoType MTBDRplus (referred to as GenoType MTBDRplus V1), for the rapid detection of multidrug-resistant TB (MDR-TB) (4).

What is MTB culture test?

A culture test involves studying bacteria by growing the bacteria on different substances. This is to find out if particular bacteria are present. In the case of the TB culture test the test is to see if the TB bacteria Mycobacterium tuberculosis, are present.

Which of the following are symptoms of TB disease?

The general symptoms of TB disease include feelings of sickness or weakness, weight loss, fever, and night sweats. The symptoms of TB disease of the lungs also include coughing, chest pain, and the coughing up of blood. Symptoms of TB disease in other parts of the body depend on the area affected.

What is 1st line LPA?

What is MTB test?

The Xpert MTB/RIF assay is a new test that is revolutionizing tuberculosis (TB) control by contributing to the rapid diagnosis of TB disease and drug resistance. The test simultaneously detects Mycobacterium tuberculosis complex (MTBC) and resistance to rifampin (RIF) in less than 2 hours.

How accurate is GeneXpert test?

However PPV, NPV and Diagnostic accuracy of Gene XPERT test was 96.77%, 94.94% and 96.23% respectively. Conclusion: Hence it has been concluded through results of this study is that GeneXpert is more accurate and reliable than sputum smear microscopy in predicting pulmonary TB.

What is DMC in tuberculosis?

Designated Microscopy Centre (DMC): The most peripheral laboratory under the RNTCP network is the designated microscopy centre (DMC) which serves a population of around 100,000 (50,000 in tribal and hilly areas).

What is Mtb positive?

Results that are positive for MTBC and for RIF resistance mean that the bacteria have a high probability of resistance to RIF. This should be confirmed by additional rapid testing.

What does Mtb mean tuberculosis?

The Xpert Mycobacterium tuberculosis (MTB)/rifampicin (RIF) is a fully automated diagnostic test that allows for the detection of MTB including its RIF resistance. Although the test is used for the diagnosis of tuberculosis (TB) in sputum samples worldwide, studies using fecal specimens are scarce.