What are the components of a centrifuge?
There are two basic components to all centrifuges, the rotor assembly and the electrical motor. The rotor assembly is that part of the centrifuge which transfers the rotation motion produced by the electrical motor.
Why are two tubes needed for centrifugation?
If you are spinning more than two tubes, only the tubes directly opposite each other have to be equal in mass. At high speeds, a centrifuge can easily become unbalanced if equal masses aren’t located opposite each other in the rotor.
How do you balance 5 tubes in a centrifuge?
To balance five tubes, create one balance tube and place two sets of three tubes across from each other. To balance seven tubes, create one balance tube and place two sets of four tubes across from each other.
Are Eppendorf tubes sterile?
Risk of contamination Tubes are only sterile in closed packaging.
How does a centrifuge separate components of a solution?
Introduction. Centrifugation is a method of separating molecules having different densities by spinning them in solution around an axis (in a centrifuge rotor) at high speed. It is one of the most useful and frequently employed techniques in the molecular biology laboratory.
What are centrifuge tubes?
Centrifuge tubes are used to contain liquids during centrifugation, which separates the sample into its components by rapidly rotating it around a fixed axis. Most centrifuge tubes have conical bottoms, which help collect any solid or heavier parts of the sample being centrifuged.
What is the volume of a centrifuge tube?
5 mL, 12 mL, 15 mL, or 50 mL sizes.
What plastic are centrifuge tubes made of?
Plastic centrifuge tubes may be manufactured from polyethylene terephthalate (PET), polypropylene, PPCO (polypropylene copolymer), polycarbonate or polystyrene. Polypropylene andPPCO tubes are autoclavable and provide excellent chemical resistance combined with good mechanical strength.
Can centrifuge tubes be reused?
Usually, only centrifuge tubes made of fluorinated ethylene propylene (FEP) are durable enough to be reused. Polypropylene centrifuge tubes are recommended for single use to ensure the accuracy in the next usage.
How do you clean a centrifuge tube?
Do hand-wash tubes and bottles, using a mild detergent solution such as Beckman Solution 555™ diluted 10 to 1 with water. Rinse with distilled water, and air-dry. (Refer to the sterilization and disinfection chart below.) Do make sure all tubes and bottles are completely dry before storing them.
What is PCR tube?
PCR tubes are small tubes designed for use in polymerase chain reaction (PCR). These tubes can be manufactured from a variety of materials, and are available in different capacities and RCF ratings. Choose from autoclavable and/or RNase-, DNase- and DNA-free. Multiple colors allow for easy sample identification.
Do centrifuged red top tubes need?
Red top tubes must be allowed to clot completely (30-60 minutes) prior to centrifugation. Centrifuging the specimen yields serum. NOTE: All drug levels must be drawn in red top tubes only.
What is centrifuge tubes?
What are Falcon tubes made of?
Falcon conical tubes are made from high-clarity polypropylene. Combining strength, chemical resistance, thermal stability, and optical clarity, Falcon tubes provide reliable performance for all of your critical research applications.
What type of plastic are Falcon tubes made from?
Polypropylene Centrifuge Tubes
Falcon™ Polypropylene Centrifuge Tubes Manufactured from polypropylene for high chemical and thermal resistance. Corning™ Falcon™ Polypropylene Centrifuge Tubes are tested to withstand 7500 x g centrifugation.
What are test tubes made of?
Test tubes intended for general chemical work are usually made of glass, and for its relative resistance to heat. Tubes made from expansion-resistant glasses, mostly borosilicate glass or fused quartz, can withstand high temperatures up to several hundred degrees Celsius.