What are 4 ways to safely handle glassware in the laboratory?
When handling glass rods, tubes, or pipettes remember the following:
- Determine the holes are the correct size for the glass material.
- Never force the glass into place.
- Lubricate the hole and tube.
- When possible, wear cut resistant gloves.
- Hold the glass material with a towel.
How will you know if the glassware is safe to use?
Inspect glassware for chips and cracks before use. When you cut glass, use hand protection and fire-polish all cut surfaces. Follow these additional tips to reduce the risk of injury: Never use laboratory glassware to serve food or drinks.
How do you test cleanliness of glass in a lab?
Use ethanol for ethanol-soluble contents, followed by rinses in deionized water. Rinse with other solvents as needed, followed by ethanol and finally deionized water. If the glassware requires scrubbing, scrub with a brush using hot soapy water, rinse thoroughly with tap water, followed by rinses with deionized water.
How do you know if a laboratory glass is dirty?
A good way to confirm that your glassware is clean is to make sure that distilled water uniformly wets the surface, according to Corning. This tells you that the surface is free of grease and other contaminants that could alter the volume being measured or introduce impurities into the liquid.
How do you care for laboratory equipment and glassware?
How to clean laboratory equipment
- Carry out a daily wipe down of all equipment exteriors.
- Carry out a weekly deep clean of all equipment.
- Carry out a regular deep clean of microscopes using a 70:30 mixture of ether and alcohol – this ensures that they are sufficiently clean to yield most accurate results.
What is the simple rule of handling glassware?
When carrying glassware, be sure to: Use both hands; Never hold it by its neck; Never carry it by its side.
What are the hazards of glassware?
Warning
| Type of Glassware Injury | Example Process/Activities | Potential Risk |
|---|---|---|
| Injury from imploding glass or flying glass. (Vacuum apparatus) | Working with vacuum apparatus. | HIGH |
| Cuts when forcing glass tubing or pipettes onto rubber stoppers | Working with glass tubes or Pasteur pipettes. | Moderate Plus |
How do you remove impurities from glass?
A diluted solution of a strong acid, such as hydrochloric acid, can be used to clean glassware, usually after they were cleaned in a base bath. It will remove most of metal impurities. Excellent for cleaning glass and plastic.
What is the ideal sterilization conditions for contaminated glassware?
The most effective and used method of sterilization of glassware is dry heat treatment using hot air oven. On exposing the glassware to dry heat, the inhibition in growth occurs due to denaturation of proteins and oxidation of biomolecules.
How do you maintain glassware?
By Hand
- Always wash new glasses before first use.
- Hand wash glasses individually.
- Do not soak with other glasses to reduce the chance of mechanical shock.
- Use a suitable detergent and warm water with a non abrasive sponge or cloth.
What are the methods of cleaning a glassware?
General Procedure For All Glassware
- To remove organic residues, rinse glasware briefly with an organic solvent (acetone or ethanol).
- Use warm tap water and a brush with soapy water to scrub the inside of curved glassware.
- Remove soapsuds with deionized water to avoid harsh water stains.
How often should you check your lab equipment?
Cleaning. Keeping your lab equipment clean means that it will always be ready for use when you need it, and ensures that no impurities contaminate samples and skew data. Lab equipment should be cleaned after every use.
What is maintenance of lab equipment?
General Cleaning It’s advisable to: Carry out a daily wipe down of all equipment exteriors. Carry out a weekly deep clean of all equipment. Carry out a regular deep clean of microscopes using a 70:30 mixture of ether and alcohol – this ensures that they are sufficiently clean to yield most accurate results.
What are the measures to be taken to avoid breakage of glassware?
Brush or vacuum the bases, and replace broken boards frequently, add rubber strips to cushion the glass. Store units of equal height on the same rack. Store units on racks with proper protection for the glass. Set glass on a rack that has chips or debris on the base.
What is a glass risk assessment?
This risk assessment covers the use and storage of glassware in the laboratory. Glassware has a wide range of uses, including beakers, pipettes, vacuum equipment, etc.
Why do we need to clean glassware?
Introduction. Good laboratory technique demands clean glassware because the most carefully executed piece of work may give an erroneous result if dirty glassware is used. In all instances, glassware must be physically and chemically clean and in many cases, it must be bacteriologically clean or sterile.
How do you sterilize glassware?
Sterilization of glassware by moist heat using autoclaving is not very effective since moisture enters the glassware and may cause contamination problems. The most effective and used method of sterilization of glassware is dry heat treatment using hot air oven.
Which acid is used for cleaning glassware in the laboratory?
Safe Use of Chromic Acid If glassware becomes unduly clouded or dirty or contains coagulated organic matter, it must be cleansed with chromic acid cleaning solution.
How do you decontaminate glassware?
Decontaminate the glassware by presoaking in 5% bleach or by boiling.
- Can use any detergent or cleaning powder.
- If the glassware is soaked in water after use, that is ideal.
- If leftover, then keeps in the detergent solution overnight.
- Now rinse with tap water, followed by a rinse with deionized water.
What is glass maintenance?
Glass is a simple, stain-proof, wipe-down surface. We recommend cleaning by spraying Windex on a paper towel and then wiping the countertop. Towel-dry after each use to avoid water spots. Do not use abrasive cleaners, Fantastic, or 409; they will create a haze on the glass.
How do you sterilize contaminated glassware?
in a disinfectant solution and then steam autoclaved then followed by a suitable washing and rinsing. Ultrasonic Cleaners Ultrasonics is a good method of cleaning glassware. generally with a mild detergent they will clean most residues off of glassware.
What are the safety rules for glassware in a lab?
Follow all lab safety rules when using and handling glassware to avoid accidents and injury. Inspection All glassware should be inspected for cracks and contamination before use. Cracked items should be disposed of, and contaminated glassware should be cleaned.
What are the glassware standards?
There are a variety of glassware standards in operation throughout the pre-production and production stages of manufacturing from evaluating the chemical, physical and mechanical properties to ensuring the glassware standards help material companies and product manufacturers in the correct fabrication and treatment procedures.
What should I do if my glassware is cracked?
Inspection All glassware should be inspected for cracks and contamination before use. Cracked items should be disposed of, and contaminated glassware should be cleaned. Disposal